TABLE 1.

Organisms isolated from the Henderson molybdenum mine

Isolate	Accession no.	Sample source (depth [ft], location, type of precipitate)	Isolation mediumb	Isolation temp (°C)	Temp (°C) optimum(a)	Mn oxidationa	Amplifiable mnxG	Closest relative (% identity)
HM06-01	EU004563	7,025, borehole 4	K-asw	50	Not tested	++	No	Bacillus firmus B563 (100)
HM06-02	EU004564	7,025, fracture, Mn oxides	K-asw	25	37	+	Yes	Bacillus sp. strain PL-12 (99)
HM06-02 mnxG	EU004573							mnxG Bacillus sp. strain PL-16 (98)
HM06-03	EU004565	7,025, borehole 1, Fe oxides	Leptothrix	50	Not tested	−	No	Paenibacillus sp. strain Smarlab 3301786 (99)
HM06-04	EU004566	7,025, fracture, Mn oxides	K-asw	25	37	+++	No	Bacillus sp. strain SD521 (99)
HM06-05	EU004567	7,025, borehole 1, Fe oxides	Leptothrix	50	Not tested	−	No	Paenibacillus sp. strain Smarlab 3301786 (99)
HM06-06	EU004568	7,025, borehole 3, Fe oxides	Leptothrix	50	Not detected	++	No	Bacillus sp. strain B-3 (99)
HM06-07	EU004569	7,025, borehole 3, Fe oxides	Leptothrix	50	Not tested	−	No	Bacillus sp. strain B-3 (100)
HM06-08	EU004570	7,025, borehole 4, Mn oxides	K-asw	50	25	++	No	Bacillus firmus B563 (100)
HM06-09	EU004571	7,025, borehole 4, Mn oxides	K-asw	50	Not tested	++	No	Bacillus firmus B563 (100)
HM06-10	EU004572	7,025, fracture, Mn oxides	K-asw	25	25 and 37	+	No	Bacillus sp. strain 2b-2 (98)
HM06-11	EU525854	7,025, borehole 4, Mn oxides	Leptothrix	50	Not tested	++	No	Georgenia sp. strain 3A-1 (95)

^a+, weak; ++, medium; +++, strong; −, none.

^basw, artificial seawater.