Figure 2.

Effects of cannabidiol (CBD) on lipopolysaccharide (LPS)-induced morphological changes in rat retinal microglial cells. Confocal micrographs were made of cells identified by microglial- and macrophage-specific marker CD11b followed by Oregon green-conjugated secondary antibody. Cell nuclei were counter-stained by propidium iodide. A: Cells were cultured in serum-free medium for 12 h. B: Cells were treated with 30 ng/ml lipopolysaccharide in serum-free medium for 12 h. C: Cells were cannabidiol (CBD, 1 μM)-pretreated and lipopolysaccharide-treated for 12 h. D-F: Cells similarly treated were also stained with fluorescent phallotoxins for Filamentous actin distribution and cellular morphology. Scale bar represents 20 μm.