Table 1. Primer sequences and the sizes of their corresponding PCR products.
| PITX2 exon | Sequences (5′-3′) | Amplicon (bp) |
|---|---|---|
|
PITX2-exon 1 |
F: CCGCTTCTTACAGCCTTCCT |
247 |
| |
R: CTGGCGATTTGGTTCTGATT |
|
|
PITX2-exon 2 |
F: TGGGTCTTTGCTCTTTGTCC |
400 |
| |
R: GCGGAGTGTCTAAGTTCAAGC |
|
|
PITX2-exon 3 |
F: GGGGCAGTAGCCAAGGACT |
289 |
| |
R: CAGCTAAGCGGGAATGTCTG |
|
|
PITX2-exon 4 |
F: GGCATGCTGACGGGAAAG |
300 |
| |
R: CTGTACCTCCACAACATCCTC |
|
|
PITX2-exon 5 (region 1) |
F: CACTGTGGCATCTGTTTGCT |
324 |
| |
R: GGACGACATGCTCATGGAC |
|
|
PITX2-exon 5 (region 2) |
F: TATGAACGTCAACCCCCTGT |
400 |
| R: CCATCCGGCAAGGTCCTA |