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. 2008 Nov 28;14:2148–2156.

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This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Immunofluorescence staining of cryosections from 6 day cocultured explants. Antibodies against glial fibrillary acidic protein (GFAP; red) and cellular retinaldehyde-binding protein (CRALBP; green) were used. Confocal images revealed an increased expression of GFAP and a decreased CRALBP expression in the neuroretinal external layers. Müller cell processes crossed the outer limiting membrane (OLM; arrows). The cell bodies of GFAP+ astrocytes remained in the nerve fiber layer (NFL; asterisk), but the extensions crossed the neuroretina, reaching the outer layers (arrowheads). Scale bar equals 20 µm. Abbreviations: ganglion cell layer (GCL); inner limiting membrane (ILM); inner nuclear layer (INL); outer nuclear layer (ONL).