Table 1. Distribution of cultured explants.
Culture days |
Control explants |
Explants+MNF |
||
---|---|---|---|---|
Cultured | Lost | Cultured | Lost | |
3 |
25 |
1 |
13 |
0 |
6 |
25 |
3 |
13 |
1 |
9 |
25 |
4 |
13 |
1 |
75 |
8 |
39 |
2 |
|
Total: 114 explants |
Control and MNF-stimulated neuroretinal explants were cultured during 3, 6 and 9 days. A total of 114 explants were used initially. Lost explants were disposed of in the experiment due to an opacification in the first days of culture. Mononuclear fraction (MNF) was added to explants in culture at day 0.