Figure 3 Predicted secondary structure analysis of wild type and mutant 5′‐UTR of MeCP2_e2 using Mfold. (A) The five most stable secondary structure predictions of the wild type MeCP2_e2 5′‐UTRs are shown in panels i–v. (B) Panels i–iv show the four most stable secondary structures predicted for the mutant MeCP2_e2 5′‐UTR, including structure 4. Panel v shows predicted structure 14. Simplified tree graphs drawn for each structure are depicted as insets. The Y shaped structure is indicated in bold within the insets. Only structures 4 and 14 of the mutant sequence show similarity to the tree graphs of the wild type structures with subtle differences that are discussed in the text.