E. coli
strains |
|
|
i484 |
O25:H autoagglutinating, human
isolate |
17 |
oy077 |
i484, fitR :: EZ-TN5<
Kan-2 > |
This study |
oy016 |
i484, lacZ :: EZ-TN5<
DHFR-1 > |
This study |
oy021 |
oy016 carrying pMP-fitB |
This study |
oy022 |
oy016 carrying pMP-fitA |
This study |
oy026 |
oy016, fitR :: EZ-TN5<
Kan-2> |
This study |
oy028 |
oy026 carrying pMP-fitB |
This study |
oy029 |
oy026 carrying pMP-fitA |
This study |
oy071 |
i484, fur :: EZ-TN5 <DHFR> |
This study |
oy078 |
oy077, fur :: EZ-TN5<DHFR> |
This study |
DH5α |
F–, ф80d lacZ∆M15 ∆
(lacZYA–argF) U169, endA1, recA1, hsdS17 (rK–mK+), deoR, thi–1, supE44,
λ–, gyrA96, relA1 |
Invitrogen |
|
|
|
Plasmids |
|
|
pMP220 |
Contains a promoterless lacZ
gene, Tetr
|
19 |
pMP–fitA
|
fit
promoter was cloned into pMP220 with a direction of fitA
transcription |
2 |
pMP–fitB
|
fit
promoter was cloned into pMP220 with a direction of fitB
transcription |
2 |
pBAD22 |
overexpression vector, Ampr
|
8 |
pBAD–fitR1 |
DNA fitR1 cloned into pBAD22 at
EcoR I and Hind III sites |
This study |
pBAD–fitR2 |
DNAfitR2 cloned into
pBAD22 at EcoR I and Hind III sites |
This study |
pCR |
Cloning vector pCR-XL-TOPO |
invitrogen |
pCR64 |
DNA fitR1 cloned into pCR-XL-TOPO |
This study |