Fig. (1).

Rhodanese activities during growth on glycerol or glucose minimal medium. Strain JLD17204 (ΔglpE::FRT) was grown overnight in minimal medium with glucose. Cells were harvested by centrifugation, washed, and then diluted into medium with either glucose (circles) or glycerol (squares). The optical density at 600 nm (closed symbols) and rhodanese activity (open symbols) were monitored. Rhodanese activity was determined using intact cells harvested at the indicated time points and corresponds to the activity (units) found in the equivalent of 1 ml of cells at an OD of 2. Substrate concentrations of 50 mM thiosulfate and 50 mM cyanide were employed for these assays.