Pre-implantation embryos were stained with affinity-purified polyclonal antibodies against ZFP57 (Figures A-H). They were also incubated with Hoechst dye to illuminate genomic DNA (Figures A’-H’).
A homozygous E1.5 embryo (A or A’) and a homozygous E3.5 embryo (D or D’) were derived from the cross between homozygous female mice and homozygous male mice. Littermate E1.5 embryos (B and C) and E3.5 embryos (F and G) were derived from the crosses between heterozygous female mice and homozygous male mice.
B (or G), a homozygote (-/-).
C (or F), a heterozygote (+/-).
The wild-type E3.5 embryo (E) was derived from the cross between a wild-type female mouse and a wild-type male mouse. The heterozygous (-/+) E3.5 embryo (H) was from the cross between a homozygous female mouse and a wild-type male mouse.