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. Author manuscript; available in PMC: 2009 Sep 1.
Published in final edited form as: Cancer Res. 2008 Sep 1;68(17):6997–7005. doi: 10.1158/0008-5472.CAN-08-1178

Figure 2.

Figure 2

Down-regulation of plakoglobin by LMP1 is dependent on the IκBα regulated canonical NFκB pathway. (A) Expression and the effects of the HA-tagged IκBα super-repressor (IκBαSS32/36AA) from the pHSCG vector, on LMP1’s down-regulation of plakoglobin was analyzed by immunoblot analysis. Activation of Akt was detected by the phosphorylation of Akt (pAkt). (B) Expression and the effects of the constitutively active myristylated-Akt (myr-Akt) and LMP1 on plakoglobin levels was compared by immunoblot analysis. Actin was used as a loading control. Densitometry with Image J software was performed to normalize pAkt and plakoglobin levels to the corresponding actin levels. Fold change of the normalized pAkt and plakoglobin levels relative to the pBabe control is indicated below the immunoblots.