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. Author manuscript; available in PMC: 2009 Sep 1.
Published in final edited form as: Cancer Res. 2008 Sep 1;68(17):6997–7005. doi: 10.1158/0008-5472.CAN-08-1178

Figure 6.

Figure 6

LMP1 induced migration is blocked by restoring plakoglobin levels. (A) Stable expression of LMP1 in the pBabe vector and a myc-tagged plakoglobin (PG) or the DsRed vector control from C666-1 cells was analyzed by immunoblot analysis. Actin and GAPDH were used as loading controls. Densitometry with Image J software was performed to normalize plakoglobin levels to the corresponding GAPDH levels. Fold change of the normalized plakoglobin levels relative to the pBabe/DsRed control is indicated below the immunoblots. (B) The effects of restoring PG levels on the migration of LMP1-expressing C666-1 cells were assessed by the transwell migration assay. (C) Soft agar transformation and (D) MTS assays were performed to assess the effect of expressing a PG construct on the growth of pBabe and LMP1-expressing C666-1 cells, compared to the DsRed vector control.