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. Author manuscript; available in PMC: 2009 Aug 1.

Published in final edited form as: Mol Immunol. 2008 Jul 7;45(14):3786–3796. doi: 10.1016/j.molimm.2008.05.018

Fig. 5 — Quantitation of Vpreb1 transcripts and DNA binding of the Vpreb1 promoter by wild type and mutated EBF: mutations of flanking residues. (A) qPCR of Vpreb1 mRNA isolated from µM.2.21 cells infected to express wild type or mutated EBF. Other aspects of these experiments (n = 3) are similar to Fig.3A. (B–C) EMSA of binding of the murine Vpreb1 site 2 (B) or site 3 (C) probe by wild type and mutated EBF proteins. *Endogenous complex unrelated to EBF. All other aspects of these experiments were performed as in Fig.3D–E.

Fig. 5 — Quantitation of Vpreb1 transcripts and DNA binding of the Vpreb1 promoter by wild type and mutated EBF: mutations of flanking residues. (A) qPCR of Vpreb1 mRNA isolated from µM.2.21 cells infected to express wild type or mutated EBF. Other aspects of these experiments (n = 3) are similar to Fig.3A. (B–C) EMSA of binding of the murine Vpreb1 site 2 (B) or site 3 (C) probe by wild type and mutated EBF proteins. *Endogenous complex unrelated to EBF. All other aspects of these experiments were performed as in Fig.3D–E.