Table 1.
Summary of mIgM expression and qPCR results for mb-1 and Vpreb1 expression.
| mb-1 |
Vpreb1 |
||
|---|---|---|---|
| mIgM | qPCR | qPC | |
| Wild type | +++ | +++ | +++ |
| E149A | +++ | ++ | ++++ |
| M150A | ++ | ++ | +++ |
| C151S | +++ | +++ | +++ |
| R152A | +/− | + | + |
| T1566A | ++ | ++ | + |
| H157A | − | − | − |
| C161S | − | − | − |
| S162A | +++ | +++ | +++ |
| R163A | − | − | + |
| C164A | − | − | − |
| C165S | +++ | +++ | +++ |
| C165A | +++ | +++ | +++ |
| D166A | +++ | +++ | +++ |
| K167A/K168A | +/− | ++ | − |
| S169A | +++ | +++ | ++++ |
| C170S | − | − | − |
| N172A | +/− | + | + |
| R173A | +++ | +++ | ++ |
| N174A | ++ | ++ | + |
| E175A | ++ | +++ | ++++ |
| T176A | +++ | +++ | ++ |
| GFP only | − | − | − |
Scores for mIgM expression are as follows: (+++)>30%, (++) 29% to 25%, (+) 24% to 20%, (+/−) 19% to 15%, and (−) <10%. Wild type is set to +++ (mIgM expression of > 30%). Scores for mb-1 and Vpreb1 qPCR expression are as follows: (++++) > 1, (+++) 1 to 0.75, (++) 0.74 to 0.50, (+) 0.49 to 0.25, (−) <0.24. Wild type is set to 1 (+++).