Table 1.
mb-1 |
Vpreb1 |
||
---|---|---|---|
mIgM | qPCR | qPC | |
Wild type | +++ | +++ | +++ |
E149A | +++ | ++ | ++++ |
M150A | ++ | ++ | +++ |
C151S | +++ | +++ | +++ |
R152A | +/− | + | + |
T1566A | ++ | ++ | + |
H157A | − | − | − |
C161S | − | − | − |
S162A | +++ | +++ | +++ |
R163A | − | − | + |
C164A | − | − | − |
C165S | +++ | +++ | +++ |
C165A | +++ | +++ | +++ |
D166A | +++ | +++ | +++ |
K167A/K168A | +/− | ++ | − |
S169A | +++ | +++ | ++++ |
C170S | − | − | − |
N172A | +/− | + | + |
R173A | +++ | +++ | ++ |
N174A | ++ | ++ | + |
E175A | ++ | +++ | ++++ |
T176A | +++ | +++ | ++ |
GFP only | − | − | − |
Scores for mIgM expression are as follows: (+++)>30%, (++) 29% to 25%, (+) 24% to 20%, (+/−) 19% to 15%, and (−) <10%. Wild type is set to +++ (mIgM expression of > 30%). Scores for mb-1 and Vpreb1 qPCR expression are as follows: (++++) > 1, (+++) 1 to 0.75, (++) 0.74 to 0.50, (+) 0.49 to 0.25, (−) <0.24. Wild type is set to 1 (+++).