Location of identified M. avium subsp. paratuberculosis proteins excised from 2-D protein gels. A silver-stained 2-D electrophoretogram of M. avium subsp. paratuberculosis grown to exponential phase in modified Middlebrook 7H9 broth is presented. Protein spots that were selected as being in greater abundance or uniquely present by comparison to the electrophoretogram of M. avium subsp. avium are highlighted, and their identifications as determined by MALDI-TOF are indicated. Some spots were apparently mixtures of proteins. The spot indicated as MAP4147 also had significant hits to MAP1012c, MAP3175c, and MAP4233. Also, the spot indicated as MAP3205 had a significant hit to MAP3385, as did MAP2878c to MAP1160c. This figure was compiled by using CorelDraw 9 software.