TABLE 3.
Cohorts of samples tested by Enfer multiplex immunoassay and the Anigen lateral-flow kit and individual responses to ESAT-6, CFP-10, and MPB83 antigens
| Test | TB-positive animals (no.) | Sensitivity (%) | TB-negative animals (no.) | Specificity (%) |
|---|---|---|---|---|
| Enfer multiplex immunoassay | 522 | 93.1 | 1,489 | 98.4 |
| ESAT-6 | 522 | 40.6a | 1,489 | 86.6a |
| CFP-10 | 522 | 82.6a | 1,489 | 69.7a |
| MPB83 | 522 | 78.5a | 1,489 | 99.1a |
| Anigen lateral flow | 214 | 83.6b | 79 | 83.0c |
a
Data were extracted from the multiplex assay for these individual antigens.
b
The sensitivity of the Anigen lateral-flow kit is 90.0% compared to that of culture-confirmed positives, and it is 85.1% compared to that of skin test-confirmed positives according to the manufacturer's instructions.
c
The specificity of the Anigen lateral-flow kit is 98.6% compared to that of skin test-confirmed negative animals according to the manufacturer's instructions.