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FACS measurement of surface molecule expression, illustrated by exemplary FACS plots. Monocytes from a representative patient and control subject were cultured for 72 h in the presence of 500 ng/ml CD40L or 100 U/ml GM-CSF. The expression of CD80 and CD86 on CD14+ cells was analyzed by FACS. Black histograms, isotype-matched irrelevant monoclonal antibodies; white histograms, anti-CD80-fluorescein isothiocyanate (FITC) or anti-CD86-phycoerythrin (PE) monoclonal antibody.
