FIG. 7.

Tyrosinase production. (A) Accumulation of a protein in Δmek-1 and Δmak-1 tissues from 6-day (6d) SCM liquid and plate cultures. (B) An extracted ion chromatogram from LC-MS-MS experiments showing a precursor peptide ion (m/z 703.86, 2+) of tyrosinase that was abundant in Δmek-1 and Δmak-1 but not detectable in either the wild type (WT) or the Δmik-1 mutant. (C and D) LC-MS-MS spectra of precursor peptide ions of m/z 703.86, 2+ (C) and m/z 785.44, 2+ (D) that were specifically matched to Neurospora tyrosinase and spanned residues 58 to 69 and 425 to 441, respectively. Only y-series ions are labeled. (E) Expression of tyrosinase examined by Northern blotting. Samples containing 50 μg of total RNA were subjected to Northern blot analysis using a radiolabeled probe corresponding to the tyrosinase ORF (∼1.7 kb). 3d, 3 days; wt, wild type.