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Gal80p-YFP is concentrated in the nucleus in Δgal1 Δgal3 cells grown in the presence of galactose. Gal80p-YFP localization in the wild-type background is shown for comparison. Also shown are gal1Δ gal3Δ cells carrying the Gal80-YFP fusion construct on a high-copy-number vector (pG80Y). The presence of pG80Y results in a significant increase in the YFP signal, and short exposures are displayed (insets). Bar = 5 μm. Raff, raffinose; WT, wild type; DIC, differential interference contrast.
