FIG. 7.

Lipids made by stressed wild-type or Δdes cells, radiolabeled with [³H]palmitate between 15 and 30 min of stress. 2-DG was added to all cells. Wild-type cells were grown for 5 h before treatment, whereas the slow-growing Δdes cells were grown for 17 h before stress. Although the C₁₈(OH)-phytoceramide was not made by 5-h dual-stressed Δdes cells (not shown), it was made by 17 h dual-stressed Δdes cells. Standards (descending order) are C₁₈-phytoceramide, glucocerebrosides, and sphingosine.