TABLE 1.
Effects of biotin deprivation upon the survival of dual-stressed cells
| Strain or mutation | Percent survival (no. of colonies) in mediuma:
|
No. of colonies in controlb | |
|---|---|---|---|
| With biotin | Without biotin | ||
| Wild type | 1 (1 ± 1) | 83 (55 ± 2.1) | 66 ± 9.2 |
| cel-1 | 59 (18 ± 2.4) | 55 (16 ± 2.5) | 30 ± 5.9 |
| succ | 10 (9 ± 3) | 43 (36 ± 8) | 84 ± 15.3 (wt) |
| os-2 | 49 (22 ± 6.1) | 62 (27 ± 3.8) | 44 ± 5.1 |
| Δos-2 | 80 (43 ± 10.3) | ND | 54 ± 6.3 |
| Δdes | 0 (0) | ND | 32 ± 2.1 |
a
Number of colonies in treatment/number of colonies in control × 100. ND, not determined.
b
Number of colonies that developed at 26°C.
c
The glucose analog was 0.75% sorbose. wt, wild type.