TABLE 2.
Effects of sphingolipids upon the survival of dual-stressed cells in the absence of biotin
| Lipid | Percent survival (no. of colonies) with sphingolipid concn (μM)a
|
No. of control coloniesb | ||||
|---|---|---|---|---|---|---|
| 0 | 1 | 5 | 10 | 20 | ||
| C2-ceramide | 89 (71 ± 3.2) | 72 (58 ± 7) | 0 (0) | 0 (0) | 0 (0) | 80 ± 4.9 |
| C2-phytoceramide | 72 (64 ± 14.6) | 81 (72 ± 8) | 70 (62 ± 7.5) | 45 (40 ± 7.5) | 0 (0) | 89 ± 8.9 |
| C2-dihydroceramide | 72 (64 ± 14.6) | ND | 82 (73 ± 10.7) | 85 (76 ± 9.6) | ND | 89 ± 8.9 |
| Sphingosine | 84 (67 ± 9.1) | ND | 44 (35 ± 11.5) | 0 (0) | ND | 79 ± 3.2 |
| Phytosphingosine | 81 (63 ± 5.1) | ND | ND | 82 (64 ± 9.1) | 60 (47 ± 12) | 78 ± 1.5 |
| Dihydrosphingosine | 84 (67 ± 9.1) | ND | 79 (63 ± 9.3) | 93 (74 ± 5.5) | 58 (46 ± 3.5) | 79 ± 3.2 |
a
Number of colonies in treatment/number of colonies in control × 100. ND, not determined.
b
Number of colonies that developed at 26°C.