FIG. 8.

Conidial uptake by macrophages is not altered in ΔAfclrD. RAW 264.7 macrophages were coincubated with freshly harvested conidia of the wild-type, ΔAfclrD, and three complemented controls for 1 hour at 37°C, washed, and incubated an additional 2 hours to permit conidial phagocytosis. Cultures were fixed and stained with calcofluor white to label extracellular conidia. The percent uptake (percentage of macrophages containing one or more conidia) (A) and the average number of conidia per macrophage (of those containing conidia) (B) were calculated from triplicate wells per strain. At least 100 macrophages were counted per well. Means are presented, and error bars indicate 1 standard deviation. Data represent at least three independent experiments.