TABLE 2.
Primers used in this study
| Primer | Sequence (5′ to 3′)a | Purpose |
|---|---|---|
| RMP10 | TTAATCTAGAGCCTGATTATGACTGGGTTTATTC | Forward for AfclrD 5′ flank |
| RMP15 | AATTAGCGGCCGCTATTTTGGGTTAGCCGTGTCT | Reverse for AfclrD 5′ flank |
| RMP14 | TTAAGGGCCCCATGGATTTTCAGGGCATGT | Forward for AfclrD 3′ flank |
| RMP13 | AATTGGGCCCCGCACCTTACTTCATCGTCA | Reverse for AfclrD 3′ flank |
| RMP17 | TTAACTCGAGAACGCTGTGTAAAGCGGAGT | Forward for A. nidulans ArgB |
| RMP18 | AATTCTCGAGCCATTGCGAAACCTCAGAAG | Reverse for A. nidulans ArgB |
| RMP23 | TTCCCTTTAGCATTGGAACG | Forward for AfclrD internal probe |
| RMP24 | CTTCTCCCCACAGAGACAGC | Reverse for AfclrD internal probe |
| RMP21 | TTAAGAACTAGTCAGGCAGGCTTAATCTCAGG | Forward for AfclrD complementation |
| RMP22 | TTAAGAGAGCTCCGAGTTACAATGTTGCTGC | Reverse for AfclrD complementation |
| JP Act F | TGACAATGTTACCGTAGAGATCC | Forward for actin probe |
| JP Act R | GGAGAAGATCTGGCATCACA | Reverse for actin probe |
| JP SSM1 F | CGACTACGGAATGTCCCAC | Forward for SSM1 probe |
| JP SSM1 R | GCTCCTCAAGGCTGCGAATC | Reverse for SSM1 probe |
| JP IMD2 F | GTCACATCCAGAGATATCCAG | Forward for IMD2 probe |
| JP IMD2 R | TTGACGCCGTCGTGGAGCTC | Reverse for IMD2 probe |
| JP PPR2 F | GATGCGAAGGAAATCGAGCTC | Forward for DST1 probe |
| JP PPR2 R | CCTCCACGATTTACCACAGTTC | Reverse for DST1 probe |
| JP RTF1 F | TGATGCTGGGTCTCACCATT | Forward for RTF1 probe |
| JP RTF1 R | GGCGACATACTCTTGATCAA | Reverse for RTF1 probe |
| JP SPT3 F | AGCTTGCACGAAGCACGATG | Forward for SPT3 probe |
| JP SPT3 R | CTATCGTAGTATAGCTCCGC | Reverse for SPT3 probe |
| JP POP2 F | CATAGGATACGGAGTTCCC | Forward for POP2 probe |
| JP POP2 R | CACCAGGAGTTAGTGCACC | Reverse for POP2 probe |
a
Restriction sites are underlined.