FIG. 3.

Levels of OCTase, Arg3 protein, and arg3⁺ transcripts in Δtrx2. The wild-type (ED668), Δtrx2 (JY31b), and Δarg3 cells with or without pREP-arg3⁺ plasmid were harvested at exponential phase (OD₅₉₅ ∼1.5 in YES medium) to prepare cell crude extract and RNA. The amount of OCTase and Arg3 protein in cell extracts was measured by determining the enzyme activity (A) and by Western blotting (B). (C) The arg3⁺ transcripts were detected by Northern analysis with rRNAs as loading controls. The relative amounts of OCTase, Arg3 protein, and arg3⁺-specific RNA compared to the wild-type level (set as 100) are also presented. In panel A, the relative enzyme activities in strains with pREP-arg3⁺ plasmid are presented compared to the wild-type background. The specific enzyme activities are shown at the top. Average values with standard deviations from four (A and C) or three (B) independent experiments are given. WT, wild type.