Schematic map of the inserts of some of the plasmids used. The map of pSI4 also represents pSI4-106; the map of pSin5 also represents pSin10-19, pSin10-106, and pSin10-5mut; and the map of pMSin-RSau also represents pMSin(V173L)-RSau. Only restriction sites used to make the different constructs are shown. The NruI site shown in square brackets was lost during construction of pMSin-RSau. Continuous line, insert; broken line, pUC19 vector; dotted line, pOK12 vector.