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. 2008 Oct 10;190(24):8003–8008. doi: 10.1128/JB.00754-08

FIG. 2.

FIG. 2.

Methylation status of plasmids tested by digestion with methylation-sensitive restriction enzymes and electrophoresis in a 1% agarose gel. Lanes 1 to 3, pSin5(WT); lanes 4 to 6, pSin10-19(N172S); lanes 7 to 9, pSin10-106(V173L); lanes 10 to 12, pSin10-5mut(A34V+K44Q+M66T+L214S+Y229H); lanes 13 to 15, pSau2; lane 16, DNA fragment size marker (Fermentas). Lanes 1, 4, 7, 10, and 13, undigested; lanes 2, 5, 8, 11, and 14, digested with Eco47I (GGWCC); lanes 3, 6, 9, 12, and 15, digested with Cfr13I (GGNCC). The plasmids pSin5, pSin10-19, pSin10-106, and pSin10-5mut contain two GGWCC and eight GGSCC sites. GGWCC and GGNCC sites with C5-methylated internal cytosines (underlined) are resistant to Eco47I and Cfr13I, respectively.