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Sau96I endonuclease activity in the crude extract of E. coli DH10B(pJAT13araE, pSau2, pOB-RSau96I) grown and induced with 0.005% arabinose as described in Materials and Methods. Agarose gel electrophoresis of lambda phage DNA digested with different dilutions of the cell extract is shown. Lanes: 1, undigested; 2, 10× diluted extract; 3, 100× diluted; 4, 1,000× diluted.
