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PQS effect on denitrifying gene expression. C23O activity was measured in cells cultured for 6 h (mid-logarithmic phase). C23O activity (nanomoles of product/minute/milligram of protein) of denitrifying gene promoters was measured and was normalized by the C23O activity of promoterless control plasmids. +PQS, cultured with exogenous PQS; −PQS, cultured without exogenous PQS. The data displayed are the means ± standard deviations of more than three independent experiments.
