TABLE 1.
Bacterial strains, plasmids, and primers
| Strains, plasmids, and primers | Relevant characteristics | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| JM109 | E. coli strain for transformation | TaKaRa |
| DH5α | E. coli strain for transformation | TaKaRa |
| S17-1 | Mobilizer strain | 48 |
| P. aeruginosa | ||
| PAO1 | WT | 27 |
| ΔpqsA | PAO1 mutant with a deletion in the pqsA gene | This study |
| ΔpqsH | PAO1 mutant with a deletion in the pqsH gene | This study |
| ΔpqsR | PAO1 mutant with a deletion in the pqsR gene | This study |
| ΔlasI | PAO1 mutant with a deletion in the lasI gene | 52 |
| ΔrhlI | PAO1 mutant with a deletion in the rhlI gene | 52 |
| ΔpqsA ΔpqsR | PAO1 mutant with deletions in the pqsA and pqsR genes | This study |
| ΔpqsA ΔpqsE | PAO1 mutant with deletions in the pqsA and pqsE genes | This study |
| ΔpqsA ΔlasI | PAO1 mutant with deletions in the pqsA and lasI genes | This study |
| ΔpqsA ΔrhlI | PAO1 mutant with deletions in the pqsA and rhlI genes | This study |
| Plasmids | ||
| pHSG398 | Cloning vector; Cpr | TaKaRa |
| pG19II | pK19mobsac derived suicide vector; sacB Gmr | 36 |
| pG19pqsA | pqsA deletion cassette in pG19II | This study |
| pG19pqsR | pqsR deletion cassette in pG19II | This study |
| pG19pqsE | pqsE deletion cassette in pG19II | This study |
| pG19pqsH | pqsH deletion cassette in pG19II | This study |
| pUCP24 | Broad-host-range vector; Gmr | 55 |
| pUCPpqsR | pqsR in pUCP24 | This study |
| pUCPpqsE | pqsE in pUCP24 | This study |
| pMEX9 | pME4510 derived promoter-probe vector; xylE Gmr | 52 |
| pMEXpqsA | pqsA promoter region in pMEX9 | This study |
| pMEXnarK | nark1 promoter region in pMEX9 | 52 |
| pMEXnirS | nirS promoter region in pMEX9 | 52 |
| pMEXnorC | norC promoter region in pMEX9 | 52 |
| pMEXnosR | nosR promoter region in pMEX9 | 52 |
| Primers | ||
| pmpqsAF3 | 5′-GGAATTCGGGGCATGTAGGTGTCCTCTTCGG-3′ | |
| pmpqsAR | 5′-CCCAAGCTTGCGGGACTTGGGATTGATCACG-3′ | |
| ΔpqsAF1 | 5′-GGTCTAGAGGCAAGGTGCAACAATGGACAGTGG-3′ | |
| ΔpqsAR1 | 5′-GACTAGTGACAGAACGTTCCCTCTTCAGCG-3′ | |
| ΔpqsAF2 | 5′-CACTAGTGAGGAACGGGCATGTTGATTCAGGC-3′ | |
| ΔpqsAR2 | 5′-GCGAAGCTTGGAAGTTCACAGGTGATCGCTGCC-3′ | |
| ΔpqsHF1 | 5′-CCCAAGCTTCTTGTCCTGCAGGTCGATATCC-3′ | |
| ΔpqsHR1 | 5′-GAAGATCTCGGTCATCCGTTGCTCCTTAGC-3′ | |
| ΔpqsHF2 | 5′-GAAGATCTTTCACCGGTGTCGGTGAGATGG-3′ | |
| ΔpqsHR2 | 5′-GCTCTAGATCGAGAGCTTCTCGAAGATGCG-3′ | |
| ΔpqsRF1 | 5′-CCCAAGCTTGCCCACCAGTACAGCATTTTGCG-3′ | |
| ΔpqsRR1 | 5′-GAAGATCTTCCTGCGCTTTCTCGAAAGCGC-3′ | |
| ΔpqsRF2 | 5′-GAAGATCTCCCTTATTCCTTTTATTGGGTGGC-3′ | |
| ΔpqsRR2 | 5′-GCTCTAGAGGCAGTACGAAGATAGCTATCGC-3′ | |
| ΔpqsEF1 | 5′-CCCAAGCTTGTAGGGCGCATCAATACGTCGG-3′ | |
| ΔpqsER1 | 5′-GAAGATCTGGTCATCATCCAGTTGACCGG-3′ | |
| ΔpqsEF2 | 5′-GAAGATCTGCCGGATGCTGGAGATTCTCTCCC-3′ | |
| ΔpqsER2 | 5′-TGCTCTAGACAGGTCGACCTGGTAGTTGCC-3′ | |
| cpqsRF | 5′-GCTCTAGAACCCAATAAAAGGAATAAGGGATGC-3′ | |
| cpqsRR | 5′-CCCAAGCTTGAACGCTCTACTCTGGTGCGG-3′ | |
| cpqsEF | 5′-GCTCTAGACGCAAACCTGAGGAGGTGAACC-3′ | |
| cpqsER | 5′-CCCAAGCTTGTCCCGTCTCAGTCCAGAGGC-3′ |