TABLE 5.
Expression of hpxD-lacZ and hpxP-lacZ promoter fusions in hpxD or hpxO mutants grown in glucose nitrogen-limiting medium in the presence or absence of either hypoxanthine or uric acid
| Promoter fusion | β-Galactosidase sp act (U/mg) from cells grown ina:
|
|||
|---|---|---|---|---|
| GGlnHx
|
GGlnUr
|
|||
| KC2653 | JA-K10 (hpxD) | KC2653 | JA-K14 (hpxO) | |
| Φ(hpxD-lacZ) | 23,609 | 32,274 | 463 | 197 |
| Φ(hpxP-lacZ) | 1,944 | 266 | 1,108 | 910 |
a
Activity values are the averages from four assays with a standard error of 15% or less. One unit of β-galactosidase activity corresponds to the amount of enzyme that hydrolyzes 1 nmol of ONPG per min. GGlnHx and GGlnUr, nitrogen-limiting media that contain 0.4% glucose and 0.04% l-glutamine plus 0.1% hypoxanthine or 0.1% uric acid, respectively.