RT-PCR analysis of P. gingivalis W83 and P. gingivalis FLL144. DNase-treated total RNAs extracted from P. gingivalis strains W83 and FLL144 were subjected to RT-PCR. Lanes 1 and 3, primers P5 and P6 (Table 2) were used to amplify the 1.2-kb uvrB gene from P. gingivalis W83 and FLL144, respectively; lanes 2 and 4, primers P7 and P8 (Table 2) were used to amplify the 0.9-kb vimA gene from P. gingivalis W83 and FLL144, respectively; lanes 5 to 8, no-RT negative controls for uvrB and vimA from P. gingivalis W83 and FLL144. All lanes contained 10 μl of amplified mixture.