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. 2008 Oct 10;190(24):7985–7993. doi: 10.1128/JB.00919-08

FIG. 6.

FIG. 6.

8-oxo-G and uracil removal activities of P. gingivalis strain W83 and FLL144 cell extracts. [γ-32P]ATP-5′-end-labeled oligonucleotide (O1) was incubated with P. gingivalis extracts for 1 h (Fpg) or 1 min (Ung), electrophoresed, and visualized. (A) Lane −, negative control containing O1; lane +, positive control containing O1 and Fpg enzyme; lane 1, O1 and treated P. gingivalis W83 extract; lane 2, O1 and treated P. gingivalis FLL144 extract; lane 3, O1 and P. gingivalis W83 extract; and lane 4, O1 and P. gingivalis FLL144 extract. (B) [γ-32P]ATP-5′-end-labeled oligonucleotides (O3 and O4) were incubated with P. gingivalis extracts for 20 min, electrophoresed, and visualized. Lane −, negative control containing O4 and Fpg enzyme; lane +, positive control containing O3 and Fpg enzyme; lane 1, O4 and treated P. gingivalis W83 extract; lane 2, O3 and treated P. gingivalis W83 extract; lane 3, O4 and treated P. gingivalis FLL144; and lane 4, O3 and treated P. gingivalis FLL144 extract. (C) Lane +, positive control containing O2 and Ung enzymes; lane 1, O2 and P. gingivalis W83 extract; and lane 2, O2 and P. gingivalis FLL144 extract.