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Restriction map and PCR verification of the cruH mutant of Synechococcus sp. strain PCC 7002. (A) Restriction map showing the construction of the cruH mutant of Synechococcus sp. strain PCC 7002. (B) Agarose gel electrophoresis of amplicons from the cruH locus for the wild type (WT) and a cruH::aadA mutant (lane 1). The data show that the cruH and cruH::aadA alleles are fully segregated in the mutant strain.
