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MS analysis of the novel clavam accumulated by orfC and orfD mutants of S. clavuligerus. HPLC profile of orfD mutant of S. clavuligerus (A), mass spectrum of the novel clavam peak (retention time, 3.62 min) seen in panel A (B), HPLC profile of wild-type S. clavuligerus (C), and mass spectrum of the alanylclavam peak (retention time, 3.77 min) seen in panel C (D) are shown. Abbreviations for metabolite peaks are given in legend to Fig. 3.
