FIG. 3.

(A) Transcription start sites for the PrrA-activated crtA, bchC, and bchF genes. Primers used for cloning into pUC19spf′ were used for extension as described in Materials and Methods. Lanes 1 and 2, primer extension reactions; lanes A, C, G, and T, nucleotides corresponding to sequencing of the pUC19spf′-based templates. Arrows show transcription start sites. (B) Upstream regions of the crtA, bchC, and bchF genes. Putative PrrA binding sites are boxed, residues corresponding to the PrrA consensus sequence (24) are shown in gray, the PpsR binding sites (30) are shown in bold, and nucleotides corresponding to transcription start sites are enlarged.