TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| Strains | ||
| R. sphaeroides strains | ||
| 2.4.1 | Wild type | Laboratory collection |
| APP11 | 2.4.1 (appA)::Tpr | 15 |
| PRRBCA2 | 2.4.1 (prrBAC)::Tpr | 33 |
| RPS1 | PRRBCA2 ppsR::Kmr | 30 |
| P. denitrificans strain | ||
| ATCC 17741 | Wild type | American Type Culture Collection |
| E. coli strains | ||
| DH5α phe | Strain used for cloning | 9 |
| S17-1 | Tra+ strain used for plasmid mobilization | 41 |
| Plasmids | ||
| pRK415 | IncP Tcr; vector | 22 |
| pECapp | pRK415::PdorC::appA | This study |
| p484-Nco1 | pRK415::appA (native PappA) | 15 |
| pRK415prrA | pRK415::prrA | This study |
| pSmNs | pRK415::ppsR | 14 |
| pSmNsABC | pSmNs::prrBAC | This study |
| pLXapp | IncQ Smr SprappA::lacZYA | This study |
| pCF200Km | IncQ Smr SprpucB1::lacZYA | 26 |
| pLX14 | IncQ Smr SprbchF::lacZYA | 17 |
| pUI2711 | IncQ Smr SprcrtA::lacZYA | 45 |
| pUC19spf′ | Vector for in vitro transcription | 1 |
| pUC19spf::bchC | pUC19spf′ plus upstream region of bchC | This study |
| pUC19spf::bchE | pUC19spf′ plus upstream region of bchE | This study |
| pUC19spf::bchF | pUC19spf′ plus upstream region of bchF | This study |
| pUC19spf::crtA | pUC19spf′ plus upstream region of crtA | This study |
| pJC412 | pUC19spf′ plus upstream region of pucB1 | 1 |
| pRKK146 | pUC19spf′ plus upstream region of cycA P2 promoter | 22 |