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. 2008 Oct 17;190(24):7975–7984. doi: 10.1128/JB.00879-08

FIG. 3.

FIG. 3.

Gel shift assays of purified HP0958 binding to the flaA transcript. (A) EMSA shows that HP0958 binds to the flaA transcript. In vitro transcripts added to incubations for EMSA were 30 ng flaA mRNA (lanes 1 to 4) and 30 ng control mRNAs (lanes 5 to 7). HP0958 protein concentrations added to EMSAs were 1,200 ng (lane 2), 3,600 ng (lanes 3 and 6), and 6,000 ng (lanes 4 and 7). (B) The flaA mRNA and HP0958 association is affected by EDTA and DTT. Lane 1, 30 ng flaA transcript; lanes 2 to 4, 30 ng flaA transcript and 6,000 ng HP0958 protein. Lanes where additional EDTA (50 mM) or DTT (10 mM) were added are labeled with a plus sign.