TABLE 3.
Comparison of results from PCR and TCID50 methods
| Virus subgroup or DNA source | Serotype | Log no. of copies/ml by PCR | R2 value | Log no. of copies/ml by TCID50 method |
|---|---|---|---|---|
| Virus subgroups | ||||
| A | 31 | 8.5 | 0.9849 | 6.6 |
| B | 7 | 9.0 | 0.9991 | 7.1 |
| C | 2 | 9.3 | 0.9950 | 8.5 |
| D | 8 | 8.5 | 0.9784 | 3.9 |
| E | 4 | 9.2 | 0.9977 | 8.4 |
| F | 41 | 9.1 | 0.9869 | 2.0 |
| Plasmid | 2 | 7.1 | 0.9996 | NAb |
a
The sensitivity of PCR for different serotypes was determined by using limiting dilutions and comparing the results to TCID50 titers expressed as log values. One serotype from each subgroup was used in both PCR and TCID50 analyses.
b
NA, not applicable.