TABLE 2.
MLST locus properties
| Gene | Primer name and sequence | Primer reference | Amplicon size(s) (bp) | Location on genomea (3.1 Mbp) | No. of alleles per locus in 61 isolatesb |
|---|---|---|---|---|---|
| plc | CPplcR; AGTTTTTCCATCCTTTGTTTTG | 16 | 544 | 49,000 | 6 |
| CPplcF; ATATGAATGGCAAAGAGGAAAC | 16 | ||||
| ddlA | CPddlA2Rb; TTGTCATACCAGGTAATGTATTT | This study | 397 | 1,004,000 | 9 |
| CPddlAF; ATAATGGGGGATCATCAGTTGC | 16 | ||||
| dut | CPdutR; CTGTAGTACCAAATCCACCACG | 16 | 441 | 843,000 | 5 |
| CPdutF; TTAAGTATTTTGATAACGCAAC | 16 | ||||
| glpK | CPglpKR; CACCTTTTGCTCCAAGGTTTGC | 16 | 574 | 2,923,000 | 6 |
| CPglpKF; TGGGTTGAGCATGATCCAATGG | 16 | ||||
| gmk | CPgmkR; TACTGCATCTTCTACATTATCG | 16 | 475 | 2,025,000 | 5 |
| CPgmkF; TAAGGGAACTATTTGTAAAGCC | 16 | ||||
| recA | CPrecAR; CTCCATATGAGAACCAAGCTCC | 16 | 475 | 1,949,000 | 4 |
| CPrecAF; GCTATAGATGTTTTAGTTGTGG | 16 | ||||
| sod | CPsodR; AATAATAAGCATGTTCCCAAAC | 16 | 478 | 1,444,000 | 5 |
| CPsodF; GATGCTTTAGAGCCATCAATAG | 16 | ||||
| tpiA | CPtpiR; CATTAGCTTGGTCTGAAGTAGC | 16 | 451 | 1,542,000 | 9 |
| CPtpiF; AAATGTGAAGTTGTTGTTTGCC | 16 | ||||
| pfoS | CPpfoSR; GCAAATCTTATTTGGAACAT | This study | 560, 562, 563 | 1,190,000 | 4 |
| CPpfoSF; GTGCAGTTGCAACCACTGTT | 24 |
a
Gene locations were based on the published genome sequence of C. perfringens strain 13 (27).
b
The average number of alleles per locus in 61 isolates was 5.9.