TABLE 2.
Concordance of sequencing for integrase inhibitor mutations of a single subtype B sample
| Sequencing run | No. of variations in 409-bp stretch | Concordance (%) | Homology to subtype B consensus sequence (%) | Sequence variation(s) at indicated nucleotide position(s)a |
|---|---|---|---|---|
| 1 | 0 | 100.0 | 98.0 | |
| 2 | 1 | 99.7 | 97.8 | 395 T→Y |
| 3 | 3 | 99.3 | 97.6 | 126 A→R, 180 T→Y, 321 C→Yb |
| 4 | 3 | 99.3 | 97.6 | 126 A→R, 180 T→Y, 321 C→Yb |
| 5 | 4 | 99.0 | 97.3 | 180 T→Y, 321 C→Y,b 392 T→Y, 395 T→Y |
| 6 | 4 | 99.0 | 96.8 | 126 A→R, 180 T→Y, 321 C→Y,b 392 T→Y |
| 7 | 5 | 98.8 | 96.8 | 126 A→R, 180 T→Y, 213 A→R, 321 C→Y,b 392 T→Y |
| 8 | 7 | 98.3 | 96.6 | 57 A→R, 84 A→R, 1267 A→R, 180 T→Y, 258 T→Y, 321 C→Y,b 372 A→R |
a
Numbers indicate nucleotide positions on the 409-bp sequencing stretch. Nucleotide exchanges were not detected. At the indicated positions, wobble nucleotides Y and R were observed. A, adenine; C, cytosine; T, thymine; Y, thymine or cytosine; R, adenine or guanine.
b
T at position 321 represents a silent mutation (corresponding to resistance mutation codon S147G).