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Amplification and standard curves for CMV gB quantification with the RT-GQ-PCR. CMV DNA in serial dilutions ranging from 1.0E + 01 to 1.0E + 09 copies was amplified in the RT-GQ-PCR. Fluorescence intensity (Rn) is plotted against the number of cycles. Slope = −3.42; intercept = 38.026; R2 = 0.9985.
