TABLE 1.
Primers and probes for gB genotypes
| Genotype, accession no., and polaritya | Sequence (5′-3′) | Position (nt)b | Amplicon size (bp) |
|---|---|---|---|
| gB1 (HS5GLYBG), M60929 | |||
| F+ | CATACGACGTCTGCTGCTCACT | 22 (121-142) | 72 |
| R− | GCTGACCGTTTGGGAAGAAG | 20 (144-168) | |
| Probe+ | TCGATCCGGTTCAGTCTCT | 19 (173-192) | |
| gB2 (HS5GLXBI), M60931 | |||
| F+ | TCTTTGGTGGAATTGGAACGT | 21 (1312-1332) | 79 |
| R− | TGTCACTCGTACTTCTTCTGGTCCTA | 26 (1365-1390) | |
| Probe+ | ATCCAGTCTGAATATCA | 17 (1334-1363) | |
| gB3 (HS5GLYBM), M85228 | |||
| F+ | TGTTGGAACTGGAACGTTTGG | 21 (1316-1336) | 72 |
| R− | TGCCCGTACTTCTCTTGGTTCT | 22 (1366-1387) | |
| Probe+ | CGGTGTGAACTCCA | 14 (1340-1364) | |
| gB4 (HS5GLYBM), M60926 | |||
| F+ | AAACGTGTCCGTCTTCGAAACT | 22 (1242-1263) | 75 |
| R− | TCCACCAGAGATTTTTGCTTGA | 22 (1295-1316) | |
| Probe+ | CCGGCGGACTAGTAGT | 16 (1265-1290) |
a
F, forward; R, reverse.
b
nt, nucleotide.