TABLE 2.
Comparison of sensitivity between LC RT-QPCR and RT-GQ-PCR
| Genotype (clinical sample name) and 10-fold dilution | Resulta (CT) obtained with:
|
|
|---|---|---|
| LC RT-QPCR (routine diagnostic assay) | RT-GQ-PCR (present study)a | |
| gB1 (AH13) | ||
| Undiluted | Pos (24.95) | Pos (26.31) |
| 10−1 | Pos (27.72) | Pos (29.73) |
| 10−2 | Pos (31.71) | Pos (33.75) |
| 10−3 | Pos (33.81) | Pos (38.94) |
| 10−4 | Pos (34.32) | Pos (39.37) |
| gB2 (AH27) | ||
| Undiluted | Pos (27.51) | Pos (30.33) |
| 10−1 | Pos (30.9) | Pos (34.10) |
| 10−2 | Pos (33.17) | Pos (37.25) |
| 10−3 | Neg | Neg |
| 10−4 | Neg | Neg |
| gB3 (AH14) | ||
| Undiluted | Pos (26.93) | Pos (28.30) |
| 10−1 | Pos (30.25) | Pos (31.81) |
| 10−2 | Pos (33.28) | Pos (35.52) |
| 10−3 | Neg | Pos (39.11) |
| 10−4 | Neg | Neg |
| gB4 (AH1) | ||
| Undiluted | Pos (31.44) | Pos (27.99) |
| 10−1 | Pos (31.88) | Pos (32.11) |
| 10−2 | Pos (34.25) | Pos (35.06) |
| 10−3 | Neg | Neg |
| 10−4 | Neg | Neg |
a
Pos, positive; Neg, negative.