TABLE 2.

Comparison of sensitivity between LC RT-QPCR and RT-GQ-PCR

Genotype (clinical sample name) and 10-fold dilution	Resulta (CT) obtained with:
	LC RT-QPCR (routine diagnostic assay)	RT-GQ-PCR (present study)a
gB1 (AH13)
Undiluted	Pos (24.95)	Pos (26.31)
10−1	Pos (27.72)	Pos (29.73)
10−2	Pos (31.71)	Pos (33.75)
10−3	Pos (33.81)	Pos (38.94)
10−4	Pos (34.32)	Pos (39.37)
gB2 (AH27)
Undiluted	Pos (27.51)	Pos (30.33)
10−1	Pos (30.9)	Pos (34.10)
10−2	Pos (33.17)	Pos (37.25)
10−3	Neg	Neg
10−4	Neg	Neg
gB3 (AH14)
Undiluted	Pos (26.93)	Pos (28.30)
10−1	Pos (30.25)	Pos (31.81)
10−2	Pos (33.28)	Pos (35.52)
10−3	Neg	Pos (39.11)
10−4	Neg	Neg
gB4 (AH1)
Undiluted	Pos (31.44)	Pos (27.99)
10−1	Pos (31.88)	Pos (32.11)
10−2	Pos (34.25)	Pos (35.06)
10−3	Neg	Neg
10−4	Neg	Neg

^aPos, positive; Neg, negative.