TABLE 2.

Resolution of discrepant genus assay-positive, culture-negative results

Mycobacterium genus assay result (OD660a)	Identification based on 16S rRNA gene sequence	Assay result		Specimen	Comment
		Smear	Culture
Positive (2.344)	M. kansasii/gastri	Positive	No growth	Sputum	M. kansasii infectionb
Positive (1.177)	M. leprae	Positive	No growth	Biopsy	M. leprae infection
Positive (1.173)	M. leprae	Positive	No growth	Biopsy	M. leprae infection
Positive (3.935)	M. kansasii/gastri	Positive	No growth	Sputum	M. kansasii infectionb
Positive (1.335)	M. kansasii/gastri	Negative	No growth	Sputum	M. kansasii infectionb
Positive (0.604)	M. frederiksbergense	Negative	No growth	CSFd	Unknown significance
Positive (1.359)	M. smegmatis	Negative	No growth	Puncture fluid	Unknown significance
Positive (1.149)	M. fortuitum-complex	Negative	No growth	CSF	Unknown significance
Positive (1.059)	Corynebacterium sp.	Not done	No growth	Urine	False positive
Positive (0.928)	No sequencec	Negative	No growth	BALe	False positive
Positive (3.772)	No sequencec	Negative	No growth	BAL	False positive
Positive (>4.00)	No sequencec	Negative	No growth	Sputum	False positive
Positive (>4.00)	No sequencec	Negative	No growth	CSF	False positive

^aOD₆₆₀ of sample in genus assay.

^bPositive culture results for other specimens of patient.

^cNo sequence could be obtained from the amplification product.

^dCerebrospinal fluid.

^eBronchoalveolar lavage.