FIG. 4.

Functional analysis of P protein in its binding to GABARAP by the mammalian two-hybrid system. (A) Schematic representations of P protein deletions and its fusion proteins. The numbers indicate the amino acid positions of BDV P protein. VP16, transactivating domain of VP16. (B) Functional analysis of P protein in its binding to GABARAP by the mammalian two-hybrid system. HeLa cells were cotransfected with the luciferase reporter plasmid pG5luc along with pGAL4-GABARAP, pVP16, pVP16-P, pVP16-PΔN1, pVP16-PΔN2, pVP16-PΔC1, and pVP16-PΔC2 as indicated. Forty-eight hours posttransfection, cell extracts were prepared and assayed for luciferase activity. The values are expressed as means plus standard errors of the means.