FIG. 1.

Establishment of synchronous infection of VSV in PC3 cells. PC3 cells were infected at various MOIs (1 to 100 PFU/cell) for 16 h. Cells were then labeled for surface expression of G protein to indicate infected cells and analyzed by flow cytometry. (a) Histograms show different populations of cells expressing low and high levels of G protein in mock-infected PC3 cells and in PC3 cells infected at MOIs of 5 and 50 (b) The histograms were gated to analyze the population expressing high levels of G protein; the data are expressed as the percentage of cells expressing G protein at their surfaces for each condition. PC3 cells require a high MOI (50 PFU/cell) to establish a synchronous infection. (c) LNCaP cells were infected at an MOI of 10 PFU/cell and labeled for surface expression of G protein to show that when infected at this MOI, LNCaP cells are synchronously infected.