FIG. 3.

Escape of VSV from the cell surface is delayed in PC3 cells relative to LNCaP cells. Cells were infected with VSV (LNCaP cells at an MOI of 10; PC3 cells at an MOI of 50) for 90 min at 4°C. As diagrammed in panel a, cells were warmed to 37°C for various times to allow endocytosis to proceed. After the warming step, a VSV-neutralizing antibody (I1) was added to the cells, and they were transferred to 4°C for 30 min to allow antibody binding. Cells were then incubated at 37°C for 16 h, labeled for surface expression of G protein, and analyzed by flow cytometry. Data are expressed as the percentage of cells expressing G at their surfaces for each condition (b).