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Primary transcription is delayed in PC3 cells relative to LNCaP cells. Cells were infected with VSV (LNCaP cells at an MOI of 10; PC3 cells at an MOI of 50) for 2, 4, 8, and 12 h in the presence (a) and absence (b) of cycloheximide. Total RNA was harvested, and levels of VSV N cDNA were analyzed by reverse transcriptase real time PCR. N cDNA levels were compared to β-actin cDNA at each condition. Data are expressed as the ratio of N cDNA to actin cDNA.
