FIG. 8.

PC3 cells constitutively express antiviral proteins that may provide immediate protection against VSV infection. Western blotting was performed on LNCaP and PC3 cell lysates to detect the expression levels and/or activation status of MxA (a), STAT1 (b), and PKR (c) during a time course of VSV infection. Cells were infected with VSV (LNCaP cells at an MOI of 10; PC3 cells at an MOI of 50), and lysates were made at the indicated times postinfection. The controls for each Western blot were HeLa cells treated for 18 h with IFN-α and 15 min with calyculin A, and actin protein expression levels were included to demonstrate equal loading among lanes. (a) Induction of MxA protein expression was detected with an antibody against total MxA. (b) Total STAT1 protein expression and STAT1 phosphorylation status were detected using total and phospho-specific antibodies. (c) Similarly, total PKR protein expression and PKR phosphorylation status were detected using total and phospho-specific antibodies. The positive signal for phospho-PKR was indicated by a doublet of bands, which was masked in the control lane due to very strong signal strength in this sample. hpi, hours postinfection.