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. 2008 Oct 1;82(24):12020–12029. doi: 10.1128/JVI.01569-08

FIG. 1.

FIG. 1.

JFH-1 G451R has an altered dependence on SR-BI. (A) Huh-7.5 cells overexpressing SR-BI were incubated with JFH-1 wt or JFH-1 G451R for 8 h. Cells were fixed after 48 and 72 h and stained for NS5A, and the mean number of infected cells per well was determined. Infectivity is expressed relative to parental Huh-7.5 cells. (B) Huh-7.5 cells were incubated with a serial dilution of rabbit anti-SR-BI serum for 1 h prior to challenge with JFH-1 wt or JFH-1 G451R. The data are expressed as percent neutralization relative to infection of Huh-7.5 cells treated with control rabbit serum. (C) Huh-7.5 cells were inoculated with JFH-1 wt or JFH-1 G451R in the presence of 10 μg/ml HDL. Infection is expressed relative to infection in the absence of HDL. Error bars indicate standard deviation from the mean (n = 3; P = 0.0069, unpaired t test).